Rivista
/
Biologia
/
Optisk rydning af plantevæv til fluorescensbilleddannelse
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Optical Clearing of Plant Tissues for Fluorescence Imaging

Optisk rydning af plantevæv til fluorescensbilleddannelse

DOI:
10.3791/63428-v

•

04:55 min

•

January 05, 2022

•

, , , ,
1Institute of Transformative Bio-Molecules (ITbM),Nagoya University, 2Institute for Advanced Research (IAR),Nagoya University, 3Division of Biological Science, Graduate School of Science,Nagoya University, 4Department of Biological Sciences, Graduate School of Science,The University of Tokyo

Capitoli

  • 00:05Introduction
  • 00:48Fixation of Samples
  • 01:48Clearing
  • 02:21Observation
  • 02:59Results: Optical Clearing and Fluorescence Imaging of Tissues
  • 04:18Conclusion

Summary

Traduzione automatica

Traduzione automatica

Her beskrives en metode til at gøre plantevæv gennemsigtigt og samtidig opretholde stabiliteten af fluorescerende proteiner. Denne teknik letter dyb billeddannelse af ryddet plantevæv uden fysisk sektionering.

Tags

Optical ClearingFluorescence ImagingWhole-plant ImagingPlant-microbe InteractionsFixationFluorescent ProteinVacuum InfiltrationClearing SolutionClearSeePBSSilicone FrameFluorescent Microscopy

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/63428?article_type=v

Proper Care and Cleaning of the Microscope
check_url/it/63428?article_type=v

Major Components of the Light Microscope
check_url/it/63428?article_type=v

Phase Contrast and Differential Interference Contrast (DIC) Microscopy
check_url/it/63428?article_type=v

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging
check_url/it/63428?article_type=v

Fluorescence Activated Cell Sorting of Plant Protoplasts
check_url/it/63428?article_type=v

Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues
check_url/it/63428?article_type=v

Time-lapse Imaging of Mitosis After siRNA Transfection
check_url/it/63428?article_type=v

Tandem Højtryks-Frysning og Quick Freeze Substitution af plantevævene for Transmission Electron Microscopy
check_url/it/63428?article_type=v

Visualisere Stromule Frekvens med Fluorescence Microscopy
check_url/it/63428?article_type=v

ACT-PRESTO: Biologisk Tissue Clearing og immunolabeling Metoder til Volume Imaging

Read Article