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Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology
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JoVE Journal Biologia dello sviluppo
Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology

Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology

DOI:
10.3791/63431-v

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09:48 min

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February 25, 2022

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, ,
1Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, Leuven Stem Cell Institute,KU Leuven (University of Leuven)

Capitoli

  • 00:04Introduction
  • 00:53Isolation of Mouse Anterior Lobe
  • 01:57Dissociation of Pituitary into Single Cells
  • 04:28Establishment and Culturing of Anterior Lobe Derived Organoids
  • 07:31Results: Establishment and Validation of Anterior Lobe Derived Organoids
  • 09:21Conclusion

Summary

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The pituitary gland is the key regulator of the body's endocrine system. This article describes the development of organoids from the mouse pituitary as a novel 3D in vitro model to study the gland's stem cell population of which the biology and function remain poorly understood.

Tags

Keywords: OrganoidsMouse PituitaryIn Vitro ModelPituitary Stem Cell BiologyPituitary RemodelingNeonatal MaturationAgingTumor GrowthCell IsolationTrypsinDNAseCell CultureMedium

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