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检测植物-真菌相互作用中果胶的双重染色方法
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Biologia
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JoVE Journal Biologia
Double-Staining Method to Detect Pectin in Plant-Fungus Interaction
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检测植物-真菌相互作用中果胶的双重染色方法

DOI:
10.3791/63432-v

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06:39 min

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February 04, 2022

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,
1Faculty of Animal Science and Food Engineering, Department of Basic Sciences,University of São Paulo, 2Laboratory of Nuclear Instrumentation, Center of Nuclear Energy in Agriculture,University of São Paulo

Capitoli

  • 00:04Introduction
  • 00:58Sample Harvesting, Fixation, and Dehydration
  • 01:42Historesin Embedding Procedure
  • 02:23Polymerization
  • 03:24Sectioning and Double Staining Process
  • 04:27Results: Double-Staining Method to Detect Pectin in Plant-Fungus Interaction
  • 05:59Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议描述了一种检测咖啡 - 真菌相互作用中的果胶的显微镜方法。

Tags

Double-stainingPectinPlant-fungus InteractionHistopathological StudiesCoffee RustCercosporiosisKarnovsky FixativeCacodylate BufferGlycol MethacrylatePolymerization

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