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Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
DOI:

08:21 min

May 16, 2022

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Capitoli

  • 00:04Introduction
  • 00:46Soil Sampling and Dilutions
  • 02:35Growth Tests on Recalcitrant Substances
  • 05:00Qualitative Enzymatic Tests
  • 06:26Results: Isolation and Screening of Fungal Strains for the Degradation of Recalcitrant Materials
  • 07:52Conclusion

Summary

Traduzione automatica

Here, we present a protocol for screening soil biodiversity to look for fungal strains involved in the degradation of recalcitrant materials. First, fungal strains able to grow on humic acids or lignocellulose are isolated. Their activity is then tested both in enzymatic assays and on pollutants such as hydrocarbons and plastics.

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