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使用场效应生物传感技术探索分子伴侣Hsp90与其客户蛋白激酶Cdc37之间的生物分子相互作用
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
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使用场效应生物传感技术探索分子伴侣Hsp90与其客户蛋白激酶Cdc37之间的生物分子相互作用

DOI:
10.3791/63495-v

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09:39 min

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March 31, 2022

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, , , ,
1The Azrieli Faculty of Medicine in the Galilee,Bar-Ilan University, 2Asian Liver Center, Department of Surgery,Stanford University School of Medicine

Capitoli

  • 00:04Introduction
  • 01:16Chip Activation
  • 02:09Target Protein Immobilization
  • 03:09Preparing Analyte Samples and Running the Experiment
  • 06:09Analysis
  • 06:43Results: Data Files Generated from Hsp90 and Cdc37 Interaction Using FEB System (Experiment 1 and 2) and ITC Analysis
  • 08:39Conclusion

Summary

Traduzione automatica

Traduzione automatica

场效应生物传感（FEB）是一种用于检测生物分子相互作用的无标记技术。它测量通过石墨烯生物传感器的电流，结合目标被固定到该传感器。FEB技术用于评估Hsp90和Cdc37之间的生物分子相互作用，并检测到两种蛋白质之间的强相互作用。

Tags

Biomolecular InteractionMolecular Chaperone Hsp90Client Protein Kinase Cdc37Field-effect Biosensing TechnologyFEBLabel-freeCost-effectiveInteraction DetectionInhibitor TestingAutomatedUser-friendlyPharmaceuticalsBiomolecular AnalysisDrug ValidationChip FunctionalizationEDCSulfo-NHSMES BufferPBSTarget MoleculeQuench Solution

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