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有机溶剂基蛋白质沉淀，在质谱分析之前实现强大的蛋白质组纯化
JoVE Journal
Chimica
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JoVE Journal Chimica
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
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有机溶剂基蛋白质沉淀，在质谱分析之前实现强大的蛋白质组纯化

DOI:
10.3791/63503-v

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11:12 min

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February 07, 2022

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, , , , ,
1Department of Chemistry,Dalhousie University, 2Proteoform Scientific Inc.

Capitoli

  • 00:04Introduction
  • 00:55Rapid (Vial-Based) Protein Precipitation with Acetone
  • 02:27Protein Precipitation by Chloroform/Methanol/Water (CMW)
  • 04:30Protein Precipitation Using a Disposable Filtration Cartridge
  • 05:45Resolubilization of Protein Pellet with Formic Acid
  • 06:58Solid-Phase Extraction (SPE) Clean-Up
  • 08:20Results: Protein Precipitation via Vial-Based Versus Cartridge Approach
  • 10:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

本方案描述了在受控条件下的溶剂型蛋白质沉淀，以便在质谱分析之前稳定快速地回收和纯化蛋白质组样品。

Tags

Keywords: Protein PrecipitationOrganic SolventsMass SpectrometrySample PreparationProteome PurificationAcetoneSodium ChlorideMethanolChloroformCentrifugationSDSPelletVortex

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