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Developmental Biology

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JoVE Journal Developmental Biology
Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System
 

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

Article DOI: 10.3791/63509-v • 09:00 min • February 11th, 2022
February 11th, 2022 •
1, 1, 1,2, 3, 1
1Department of Biology, Reed College, 2Committee on Development, Regeneration, and Stem Cell Biology, University of Chicago, 3Department of Cell and Developmental Biology, University College London

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The article explains how to surgically remove eyes from living zebrafish larvae as the first step toward investigating how retinal input influences optic tectum growth and development. In addition, the article provides information about larval anesthetization, fixation, and brain dissection, followed by immunohistochemistry and confocal imaging.

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Keywords: Eye Removal Zebrafish Larvae Innervation-dependent Growth Visual System Retinal Input Optic Tectum Neural Development Regeneration Degeneration Surgical Technique Anesthesia Immobilization Agarose
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