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Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
 

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Article DOI: 10.3791/63510-v • 07:23 min • March 7th, 2022
March 7th, 2022 •
*1,2,3, *3, 3, 4, 5, 3, 5, 6, 1,2, 7
1Department of Cardiology, Affiliated Hospital of Nanjing University of Chinese Medicine, 2Department of Cardiology, Jiangsu Province Hospital of Chinese Medicine, 3First College of Clinical Medicine, Biological Technology Center for Innovation in Chinese Medicine, Nanjing University of Chinese Medicine, 4School of Health Preservation and Rehabilitation, Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, 5Department of Cardiology, Jiangsu Provincial Hospital of Chinese Medicine, 6Department of Cardiology, Yancheng TCM Hospital Affiliated to Nanjing University of Chinese Medicine, 7Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine (TCM) Prevention and Treatment of Tumor, Nanjing University of Chinese Medicine
* These authors contributed equally

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Myocardial ischemia-reperfusion model of rat heart is improved by using a self-made retractor, polyvinyl chloride tube, and a unique knotting method. Electrocardiogram, triphenyltetrazolium chloride and histological staining, and percent survival analysis results showed that the improved model group has higher success and survival rates than the already existing model group.

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Keywords: Myocardial Ischemia Reperfusion Injury Rodent Model Self-made Retractor Polyvinyl Chloride Tube Knotting Method Survival Rate Impact Size Anesthesia Paper Clips PVC Tube Suture Ophthalmic Scissors Tape Depilatory Cream Alcohol Iodophor Scrub Neck Muscles Trachea Tracheal Rings Suction Trocar Mechanical Ventilation Incision Pectorals Major Serratus Anterior Intercostal Space Pericardium Left Atrial Appendage Coronary Ostium
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