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实时检测和捕获共培养物中的侵袭细胞亚群
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JoVE Journal Ricerca sul cancro
Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures
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实时检测和捕获共培养物中的侵袭细胞亚群

DOI:
10.3791/63512-v

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08:00 min

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March 30, 2022

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, , , ,
1Lombardi Comprehensive Cancer Center,Georgetown University Medical Center, 2Department of Physics,Georgetown University

Capitoli

  • 00:05Introduction
  • 00:49Cell Culture
  • 01:17Cell Seeding and Assembly
  • 04:09Impedance Measurement
  • 04:44Detachment and Cell Collection
  • 05:54Results: Detection and Capture of Invasive Cell Subpopulations in Real-Time
  • 07:34Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们描述了一种实时检测和捕获侵袭性细胞亚群的方法。实验设计使用实时细胞分析，通过监测细胞电阻抗的变化。可以捕获复杂组织中的浸润性癌症，免疫，内皮或基质细胞，并且可以评估共培养物的影响。

Tags

Keywords: Real-time DetectionInvasive Cell SubpopulationsCo-culturesCell InvasionCell MotilityCell CultureCell AnalyzerCell RecoveryCell TherapyMetastasisCancer CellsStromal CellsImmune CellsHeterogeneous Cell MixesTrypsinEDTASerumCell AnalysisCell Migration

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