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Biochemistry

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JoVE Journal Biochemistry
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
 

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Article DOI: 10.3791/63519-v • 09:49 min • March 16th, 2022
March 16th, 2022 •
*1, *2, 1, 1, 1, 1, 1, 1, 1, 3
1Materials and Structural Analysis Division, Thermo Fisher Scientific, 2Max Planck Institute of Biochemistry, Molecular Structural Biology, 3Materials and Structural Analysis Division, Thermo Fisher Scientific
* These authors contributed equally

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High-resolution cryo-EM maps of macromolecules can be also achieved by using 200 kV TEM microscopes. This protocol shows the best practices for setting accurate optics alignments, data acquisition schemes, and selection of imaging areas that are all essential for the successful collection of high-resolution datasets using a 200 kV TEM.

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Keywords: Cryo-EM 200 KV TEM High-resolution Protein Structure Protein Complexes Molecular Mechanisms Structure-based Drug Design Apoferritin Proteasome Auto Grids Liquid Nitrogen Automated Data Collection Atlas EPU
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