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Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
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Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

DOI:
10.3791/63523-v

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14:12 min

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November 21, 2023

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, , , , , ,
1Shanghai Key Lab of Modern Optical System,University of Shanghai for Science and Technology, 2Graduate School of Engineering,Osaka University

Capitoli

  • 00:09Introduction
  • 02:28Fabrication of CF-PCR Microfluidic Chip
  • 04:58Preparation of the PCR Solution
  • 05:36Construction of the CF-PCR System
  • 07:03Construction of the CE System
  • 08:34Run the PCR Solution
  • 09:48Detection of the PCR Products by this CE System Built Inhouse
  • 11:15Representative Results
  • 12:53Conclusion

Summary

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This protocol describes how to build a continuous-flow-polymerase chain system based on a microfluidic chip and how to build a capillary electrophoresis system in the lab. It presents a simple method for the analysis of nucleic acids in the lab.

Tags

Continuous-flow PCRMicrofluidic ChipCapillary ElectrophoresisNucleic Acid AmplificationEscherichia ColiPhotolithographySU-8 PhotoresistSoft BakeExposurePost-exposure Bake

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