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Isolation of Primary Mouse Retinal Pigmented Epithelium Cells
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Biologia
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JoVE Journal Biologia
Isolation of Primary Mouse Retinal Pigmented Epithelium Cells

Isolation of Primary Mouse Retinal Pigmented Epithelium Cells

DOI:
10.3791/63543-v

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06:21 min

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November 04, 2022

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, , ,
1Eye Research Institute,Oakland University, 2Eye Research Center (OUWB)/ERC,William Beaumont School of Medicine, 3Oral Biology and Diagnostic Sciences, Dental College of Georgia,Augusta University

Capitoli

  • 00:01Introduction
  • 00:39Enucleation
  • 01:47Dissection
  • 04:43Centrifugation
  • 05:00Cell Culture
  • 05:15Representative Results
  • 05:58Conclusion

Summary

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This manuscript describes a simplified protocol for the isolation of retinal pigmented epithelium (RPE) cells from mouse eyes in a stepwise manner. The protocol includes the enucleation and dissection of mouse eyes, followed by the isolation, seeding, and culturing of RPE cells.

Tags

Primary Mouse Retinal Pigmented Epithelium CellsRetinal Pigmented EpitheliumOuter Blood-retinal BarrierAge-related Macular DegenerationPovidone IodineHanks’ Balanced Saline SolutionRPE Cell Growth MediaEnzyme AEnzyme BOra SerrataDissectionIsolation

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