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小鼠原代视网膜色素上皮细胞的分离

JoVE Journal
Biologia
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JoVE Journal Biologia

Isolation of Primary Mouse Retinal Pigmented Epithelium Cells

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小鼠原代视网膜色素上皮细胞的分离

DOI:

10.3791/63543-v

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06:21 min

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November 04, 2022

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Ryan Tomaszewski², Pragya Rajpurohit, Mei Cheng², Amany Tawfik^2,3

¹Eye Research Institute,Oakland University, ²Eye Research Center (OUWB)/ERC,William Beaumont School of Medicine, ³Oral Biology and Diagnostic Sciences, Dental College of Georgia,Augusta University

Capitoli

00:01Introduction
00:39Enucleation
01:47Dissection
04:43Centrifugation
05:00Cell Culture
05:15Representative Results
05:58Conclusion

Summary

Traduzione automatica

本手稿描述了一种逐步从小鼠眼睛中分离视网膜色素上皮（RPE）细胞的简化方案。该方案包括小鼠眼睛的剜除和解剖，然后是RPE细胞的分离，接种和培养。

Tags

Primary Mouse Retinal Pigmented Epithelium Cells Retinal Pigmented Epithelium Outer Blood-retinal Barrier Age-related Macular Degeneration Povidone Iodine Hanks’ Balanced Saline Solution RPE Cell Growth Media Enzyme A Enzyme B Ora Serrata Dissection Isolation

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