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Etablering af dyb hypotermisk kredsløbsstop hos rotter
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Establishment of Deep Hypothermic Circulatory Arrest in Rats

Etablering af dyb hypotermisk kredsløbsstop hos rotter

DOI:
10.3791/63571-v

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08:39 min

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December 16, 2022

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,
1Department of Cardiopulmonary Bypass, National Center for Cardiovascular Disease and Fuwai Hospital, State Key Laboratory of Cardiovascular Medicine, Chinese Academy of Medical Science,Peking Union Medical College

Capitoli

  • 00:05Introduction
  • 00:35Preparatory Work
  • 01:04Anesthesia and Cannulation
  • 02:54DHCA Initiation
  • 03:28Cooling
  • 03:55Deep Hypothermic Circulatory Arrest
  • 04:34Warm-Up and Reperfusion
  • 05:27Weaning off CPB
  • 05:59Risultati
  • 08:20Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne protokol præsenterer etableringen af dyb hypotermisk kredsløbsstop hos rotter, som kan anvendes til at undersøge systemisk inflammatorisk responssyndrom, iskæmi / reperfusionsskade, oxidativ stress, neuroinflammation osv.

Tags

Deep Hypothermic Circulatory ArrestDHCARatCardiopulmonary BypassCPBAnesthesiaCannulationFemoral ArteryTail ArteryJugular VeinHeparinPriming SolutionBlood Pressure Monitoring

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