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Rapid Genotyping of Mouse Tissue Using Sigma’s Extract-N-Amp Tissue PCR Kit
JoVE Journal
Biologia
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JoVE Journal Biologia
Rapid Genotyping of Mouse Tissue Using Sigma’s Extract-N-Amp Tissue PCR Kit

Rapid Genotyping of Mouse Tissue Using Sigma’s Extract-N-Amp Tissue PCR Kit

DOI:
10.3791/636-v

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12:39 min

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January 22, 2008

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,
1Department of Developmental and Cell Biology,University of California, Irvine (UCI)

Capitoli

  • 00:00Titolo
  • 00:12Introduction
  • 01:04Preparing Tissue and PCR Reaction Mixture
  • 06:58Real Time Quantitative PCR
  • 11:10Melting Curve Analysis
  • 11:45Conclusion

Summary

Traduzione automatica

Traduzione automatica

The complete genotyping of a mouse tail sample, including tissue digestion and PCR readout, is done in one and a half hours using Sigma's SYBR Green Extract-N-Amp Tissue PCR kit.

Tags

Rapid GenotypingDNA DetectionPCR AmplificationElectrophoretic GelGenotypePCR-ready DNAGenotyping ProcessMouse Tail SampleDNA ExtractionReal-time PCR AmplificationPositive Sample IdentificationPrompt Genotype Identification

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