È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:04Introduction
- 00:53Preparation of the 3D Culture System
- 02:20Growth of Spheroids in the Bioreactor
- 03:15Histone Extraction
- 04:27The First Round of Derivatization
- 05:03Desalting, Sample Cleanup, and Sample Preparation for LC-MS/MS
- 06:08Results: Identification and Quantification of Histone Post-Translational Modifications
- 07:37Conclusion
