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Isolation, Expansion, and Differentiation of Mesenchymal Stem Cells from the Infrapatellar Fat Pad of the Goat Stifle Joint
JoVE Journal
Bioingegneria
Author Produced
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JoVE Journal Bioingegneria
Isolation, Expansion, and Differentiation of Mesenchymal Stem Cells from the Infrapatellar Fat Pad of the Goat Stifle Joint
DOI:

12:06 min

August 02, 2022

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Capitoli

  • 00:05Introduction
  • 01:08Isolation of Infrapatellar Fat Pad Mesenchymal Stem Cells (IFP-MSCs) from the Femorotibial Joint of Goat Knee
  • 05:16Maintenance and Expansion of Isolated Cells
  • 07:13Differentiation Potential of IFP-MSCs
  • 08:49Results: Expansion, Clonogenic Ability, and Differentiation Potential of IFP-MSCs
  • 11:07Conclusion

Summary

Traduzione automatica

Infrapatellar fat pad mesenchymal stem cells (IFP-MSCs) can be isolated easily from the infrapatellar fat pad of the knee joint. They proliferate well in vitro, form CFU-F colonies, and differentiate into adipogenic, chondrogenic, and osteogenic lineages. Herein, the methodology for the isolation, expansion, and differentiation of IFP-MSCs from goat stifle joint is provided.

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