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Formulation et caractérisation d’un système de transport de dopamine à base d’exosomes
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1Vocational School of Health Services/İstanbul,Altinbas University, 2Chemical Metallurgy Faculty, Department of Bioengineering,Yildiz Technical University, 3Faculty of Medicine, Department of Histology & Embryology,Istinye University, 43D Bioprinting Design & Prototyping R&D Center,Istinye University, 5Stem Cell and Tissue Engineering R&D Center,Istinye University, 6Medicine Faculty,Istinye University, 7Vocational School of Health Services,Istinye University, 8The V. Akhundov National Scientific Research Medical Prophylactic Institute, 9Center for Stem Cells and Regenerative Medicine (LivMedCell),Liv Hospital
Capitoli
- 00:04Introduction
- 00:56Culturing and Cryopreservation of Wharton’s Jelly Mesenchymal Stem Cells
- 01:30Production of Exosomes from Wharton’s Jelly Mesenchymal Stem Cells
- 02:20Dopamine Loading into Exosomes and Characterization of Dopamine Loaded Exosomes
- 03:15Results: Development of a Nanocarrier System by Encapsulation of Dopamine into Exosomes Isolated from WJ‐MSCs
- 05:07Conclusion
Ici, nous avons cherché à obtenir une formulation par chargement dopaminergique des exosomes isolés de cellules souches issues des cellules souches mésenchymateuses gelées de Wharton. L’isolement et la caractérisation des exosomes, la charge du médicament dans les exosomes résultants et l’activité cytotoxique de la formulation développée sont décrits dans ce protocole.
