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Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess
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Biologia
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JoVE Journal Biologia
Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess

Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess

DOI:
10.3791/63646-v

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07:35 min

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June 01, 2022

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, ,
1Department of Anesthesiology,Columbia University Medical Center

Capitoli

  • 00:04Introduction
  • 00:37Mitochondrial Oxygen Consumption and Membrane Potential (ΔΨ)
  • 05:25Results: Determination of Voltage Threshold for mPTP Opening in Neonatal Fragile X Syndrome Mice
  • 07:03Conclusion

Summary

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This method exploits the contribution of the mitochondrial permeability transition pore to low-conductance proton leak to determine the voltage threshold for pore opening in neonatal fragile X syndrome mice with increased cardiomyocyte mitochondrial coenzyme Q content compared to wildtype control.

Tags

Mitochondrial Permeability Transition PoreOpen ProbabilityCoenzyme QOxygen ElectrodeTPP-selective ElectrodeCalibrationMitochondrial Membrane Potential

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