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辅酶Q过量设置下线粒体通透性转变孔隙的开放概率评估

JoVE Journal
Biologia

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JoVE Journal Biologia

Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess

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辅酶Q过量设置下线粒体通透性转变孔隙的开放概率评估

DOI:

10.3791/63646-v

•

07:35 min

•

June 01, 2022

•

Keren K. Griffiths, Aili Wang, Richard J. Levy

¹Department of Anesthesiology,Columbia University Medical Center

Capitoli

00:04Introduction
00:37Mitochondrial Oxygen Consumption and Membrane Potential (ΔΨ)
05:25Results: Determination of Voltage Threshold for mPTP Opening in Neonatal Fragile X Syndrome Mice
07:03Conclusion

Summary

Traduzione automatica

该方法利用线粒体通透性转变孔隙对低导质子泄漏的贡献，确定与野生型对照相比，心肌细胞线粒体辅酶Q含量增加的新生儿脆性X综合征小鼠孔隙开放的电压阈值。

Tags

Mitochondrial Permeability Transition Pore Open Probability Coenzyme Q Oxygen Electrode TPP-selective Electrode Calibration Mitochondrial Membrane Potential

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