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Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

DOI:
10.3791/63651-v

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10:16 min

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March 28, 2022

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1Department of Biological and Vision Sciences, College of Optometry,The State University of New York

Capitoli

  • 00:05Introduction
  • 00:48Eyeball Cleaning, Retina Extraction, Transfer, and Washing
  • 03:06Retina Dissociation
  • 05:20Cell Passage to Remove Neuronal Survivors
  • 07:12Results: Tracking of Mller Glia Conversion into Progenitor Cells and Neuronal Identity Validation
  • 09:24Conclusion

Summary

Traduzione automatica

Traduzione automatica

Müller glia primary cultures obtained from mouse retinas represent a very robust and reliable tool to study the glial conversion into retinal progenitor cells after microRNA treatment. Single molecules or combinations can be tested before their subsequent application of in vivo approaches.

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