Rivista
/
Neuroscienze
/
Fuld versus subregional kvantificering af amyloid-beta-belastning på musehjerneafsnit
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections

Fuld versus subregional kvantificering af amyloid-beta-belastning på musehjerneafsnit

DOI:
10.3791/63669-v

•

07:28 min

•

May 19, 2022

•

, , , ,
1Henry E. Riggs School of Applied Life Sciences,Keck Graduate Institute, 2Crean College of Health and Behavioral Sciences,Chapman University, 3Keck Science Department,Claremont McKenna College, 4Department of Biomedical and Pharmaceutical Sciences, School of Pharmacy,Chapman University, 5Department of Neurology,University of California, Irvine

Capitoli

  • 00:05Introduction
  • 00:42Full-Region of Interest (ROI) Analysis
  • 03:28Sub-Region of Interest (ROI) Analysis
  • 05:19Results: Impact of Size of ROI on Aβ-Positive Area Quantification in the APP/PS1 Transgenic Mouse of Alzheimer’s Disease
  • 06:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne protokol beskriver og sammenligner proceduren for at udføre en fuldregion eller underregion af interesseanalyse af sagittal musehjerneafsnit for at kvantificere amyloid-beta-belastning i APP / PS1 transgene musemodel af Alzheimers sygdom.

Tags

Amyloid-betaPlaque LoadAPP/PS1 Transgenic MiceImage AnalysisFull Region Of InterestSub-region Of InterestScale BarBrightnessContrastHippocampusCortex6-E10 StainingColor Thresholding

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/63669?article_type=v

Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices
check_url/it/63669?article_type=v

Double Fluorescence in situ Hybridization in Fresh Brain Sections
check_url/it/63669?article_type=v

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
check_url/it/63669?article_type=v

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures
check_url/it/63669?article_type=v

Morfometriske Analyser af Retina Sektioner
check_url/it/63669?article_type=v

Stereotaktisk Infusion af Oligomert amyloid-beta i musen Hippocampus
check_url/it/63669?article_type=v

Kombination Dobbelt Fluorescens<em> In Situ</em> Hybridisering med immunomærkning for Påvisning af ekspression af tre gener i musehjerne Sektioner
check_url/it/63669?article_type=v

Klargøring af muse retinale Cryo-sektioner til immun histokemi
check_url/it/63669?article_type=v

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections
check_url/it/63669?article_type=v

Kvantificering af immunostained Caspase-9 i retinalt væv

Read Article