/
/
कोशिकाओं और ऊतकों में ग्लूकोज अपटेक के अप्रत्यक्ष उपाय के रूप में बाह्य ग्लूकोज की कमी पूर्व विवो
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:04Introduction
- 01:043T3‐L1 Cell Culture and Maintenance
- 01:49Starvation of 3T3‐L1 Cells
- 02:22Preparation of FD‐Glucose Solutions with Different Concentrations
- 04:19Treating Starved 3T3‐L1 Cells
- 05:03Extracellular and Intercellular Measurements for Stimulated 3T3‐L1 Cells
- 06:00Protein‐Based Normalization of Intercellular Glucose Uptake
- 06:35Ex Vivo Measurement of Extracellular FD‐Glucose Depletion in Organs
- 07:59Results: Extracellular FD‐Glucose Correlation with Intracellular Uptake and Kinetics of Extracellular FD‐Glucose Depletion
- 09:39Conclusion
