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Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice

Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice

DOI:
10.3791/63695-v

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14:10 min

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July 29, 2022

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, , ,
1Department of Biochemistry,Indian Institute of Science, 2Center for BioSystems Science and Engineering,Indian Institute of Science, 3Undergraduate Program,Indian Institute of Science

Capitoli

  • 00:03Introduction
  • 02:20Culture Preparation of Salmonella Typhimurium
  • 05:15Mice and Infections
  • 05:56CFU Assessment of Organs
  • 09:06Flow Cytometric Analysis of Various Immune Cell Populations in Peritoneal Exudate
  • 11:56Risultati
  • 13:22Conclusion

Summary

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This protocol describes the induction of Gram-negative monobacterial sepsis in a mouse model system. The model is useful in investigating the inflammatory and lethal host responses during sepsis.

Tags

Salmonella TyphimuriumSeptic PeritonitisSepsisMice ModelGram-negative InfectionBacterial BurdenBSL-2LB BrothSalmonella Shigella Agar

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