Studying Murine Small Bowel Mechanosensing of Luminal Particulates

Arnaldo Mercado-Perez; Andrew Wegner; Kaitlyn Knutson; Michael Zumchak; Arthur Beyder

doi:10.3791/63697

Rivista

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Medicina

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Studying Murine Small Bowel Mechanosensing of Luminal Particulates

JoVE Journal
Medicina

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JoVE Journal Medicina

Studying Murine Small Bowel Mechanosensing of Luminal Particulates

DOI:

10.3791/63697-v

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10:21 min

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March 18, 2022

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Arnaldo Mercado-Perez*^1,2, Andrew Wegner*¹, Kaitlyn Knutson, Michael Zumchak, Arthur Beyder⁴

¹Enteric NeuroScience Program (ENSP), Division of Gastroenterology & Hepatology, Department of Medicine,Mayo Clinic, ²Medical Scientist Training Program (MSTP),Mayo Clinic, ³Easy Learning Labs, ⁴Department of Physiology & Biomedical Engineering (PBME),Mayo Clinic

Capitoli

00:05Introduction
00:49Intraluminal Content Gavage and Bowel Dissection
03:42Ex Vivo Imaging and Analysis
07:47Results: Fluorescence Traces Analysis, Geometric Center of Fluorescence Distribution, and Categorization of Power Spectra
09:32Conclusion

Summary

Traduzione automatica

To study how the small bowel handles particulates of varying sizes, we have modified an established in vivo method to determine small bowel transit.