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骨骼肌中脂质液滴含量的纤维类型和亚细胞特异性分析
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Medicina
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JoVE Journal Medicina
Fiber Type and Subcellular-Specific Analysis of Lipid Droplet Content in Skeletal Muscle
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骨骼肌中脂质液滴含量的纤维类型和亚细胞特异性分析

DOI:
10.3791/63718-v

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11:50 min

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June 08, 2022

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, , ,
1Endocrinology, Diabetes and Nutrition Unit, Institute of Experimental and Clinical Research, Medical Sector,Université Catholique de Louvain, 2Departamento de Fisiología, Facultad de Biología,Universidad de Sevilla

Capitoli

  • 00:05Introduction
  • 01:06Cryosectioning, Fiber Typing, and Bodipy Staining
  • 04:06Acquisition of Images
  • 06:12Analysis of Images
  • 09:26Results: Fiber Type and Subcellular-Specific Quantification of Lipid Droplet
  • 10:55Conclusion

Summary

Traduzione automatica

Traduzione automatica

越来越多的证据表明，脂质在骨骼肌内的过度浸润会导致脂毒性和糖尿病。在这里，我们提出了一个完整的方案，包括组织处理，使用Bodypy染色，图像采集和分析，以纤维型特定方式量化脂质液滴的大小，密度和亚细胞分布。

Tags

Fiber TypeLipid DropletSubcellular DistributionSkeletal MuscleMyosteatosisImmunohistochemistryBodipyCryosectionQuantification

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