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انعكاس التداخل المتزامن والمجهر الفلوري للانعكاس الداخلي الكلي لتصوير الأنابيب الدقيقة الديناميكية والبروتينات المرتبطة بها
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Capitoli
- 00:05Introduction
- 00:29Preparation of PDMS Channels
- 02:15Preparation for Imaging
- 03:02Growing Dynamic Guanosine Diphosphate “Extensions”
- 03:46Kinesin Motility Assay
- 04:23Image Processing and Analysis
- 05:22Results: Imaging of Dynamic Microtubules and Fluorescently Labeled Microtubule-Associated Proteins
- 05:57Conclusion
Tags
Keywords: Simultaneous Interference ReflectionTotal Internal Reflection Fluorescence MicroscopyDynamic MicrotubulesAssociated ProteinsLabel-free MicrotubulesFluorescently Labeled ProteinsPDMS PolymerMaster MoldPlasma CleaningMicrochannelsIRM ImagingFluorescent MicrobeadsMicrotubule Extension Mixture
