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同步干涉反射和全内反射荧光显微镜，用于动态微管和相关蛋白质的成像

JoVE Journal
Biochimica

This content is Free Access.

JoVE Journal Biochimica

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

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同步干涉反射和全内反射荧光显微镜，用于动态微管和相关蛋白质的成像

DOI:

10.3791/63730-v

•

06:43 min

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May 03, 2022

•

Yazgan Tuna, Amer Al-Hiyasat, Jonathon Howard²

¹Department of Molecular Biophysics & Biochemistry,Yale University, ²Department of Physics,Yale University

Capitoli

00:05Introduction
00:29Preparation of PDMS Channels
02:15Preparation for Imaging
03:02Growing Dynamic Guanosine Diphosphate “Extensions”
03:46Kinesin Motility Assay
04:23Image Processing and Analysis
05:22Results: Imaging of Dynamic Microtubules and Fluorescently Labeled Microtubule-Associated Proteins
05:57Conclusion

Summary

Traduzione automatica

我们提出了一种用于实现干涉反射显微镜和全内反射荧光显微镜的方案，用于动态微管和荧光标记的微管相关蛋白的同时成像。

Tags

Keywords: Simultaneous Interference Reflection Total Internal Reflection Fluorescence Microscopy Dynamic Microtubules Associated Proteins Label-free Microtubules Fluorescently Labeled Proteins PDMS Polymer Master Mold Plasma Cleaning Microchannels IRM Imaging Fluorescent Microbeads Microtubule Extension Mixture

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