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龟科害虫的大规模饲养与分子研究
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Mass-Rearing and Molecular Studies in Tortricidae Pest Insects
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龟科害虫的大规模饲养与分子研究

DOI:
10.3791/63737-v

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06:22 min

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March 25, 2022

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, , ,
1Department of Applied Biological Science,Tokyo University of Agriculture and Technology

Capitoli

  • 00:05Introduction
  • 00:42Insect Collection and Mass Rearing
  • 01:32Separation of Eggs and Pharate Larvae from Egg Masses for Fixation, Permeabilization, Staining, and DNA and RNA Extraction from Pharate Larvae
  • 04:35Results: Morphological Observation of Embryos and Nucleic Acid Extraction
  • 05:52Conclusion

Summary

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本方案描述了实验室中Tortricid pest insects的饲养方法。区分昆虫性别和提取核酸以进行高通量测序的程序是使用两种tortricid害虫建立的。

Tags

TortricidaeMass RearingMolecular StudiesPest InsectsInsect EggsArtificial DietEgg MassSodium HypochloritePotassium HydroxideHeptaneParaformaldehydeMethanolEthanolDAPILactic AcidAcetic AcidLarval Dissection

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