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Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers
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Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

DOI:
10.3791/63758-v

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09:36 min

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July 28, 2022

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, , ,
1Department of Pediatrics, Division of Neonatal-Perinatal Medicine,University of Oklahoma Health Sciences Center

Capitoli

  • 00:04Introduction
  • 00:46Enteroid Monolayer Formation
  • 03:47Monolayer Treatment and Scratch Wound Assay
  • 06:36Scratch Wound Analysis
  • 07:38Results: Enteroid Generation and Enteroid-Derived Monolayer Wound Healing Assay Procedure, Images, Analysis, and the Effect of HA35 on Wound Healing
  • 08:58Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes a method to establish and perform a scratch wound assay on two-dimensional (2D) monolayers derived from three-dimensional (3D) enteroids isolated from non-human primate ileum.

Tags

Hyaluronic AcidPretermSmall IntestineEnteroidMonolayerWound HealingRegenerationIn Vitro ModelCell CultureExtracellular MatrixCell DissociationTrypsinDMEM/F-122D Model

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