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Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers
 

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

Article DOI: 10.3791/63758-v • 09:36 min • July 28th, 2022
July 28th, 2022 •
*1, *1, 1, 1
1Department of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Oklahoma Health Sciences Center
* These authors contributed equally

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This protocol describes a method to establish and perform a scratch wound assay on two-dimensional (2D) monolayers derived from three-dimensional (3D) enteroids isolated from non-human primate ileum.

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Keywords: Hyaluronic Acid Preterm Small Intestine Enteroid Monolayer Wound Healing Regeneration In Vitro Model Cell Culture Extracellular Matrix Cell Dissociation Trypsin DMEM/F-12 Tissue Culture 2D Model
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