/

/

从蓝藻中提取非蛋白质氨基酸用于液相色谱-串联质谱分析

JoVE Journal
Chimica

This content is Free Access.

JoVE Journal Chimica

Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis

Please note that all translations are automatically generated. Click here for the English version.

从蓝藻中提取非蛋白质氨基酸用于液相色谱-串联质谱分析

DOI:

10.3791/63779-v

•

08:14 min

•

December 09, 2022

•

Sercan Pravadali-Cekic, Jake P. Violi, Simon M. Mitrovic, Kenneth J. Rodgers, David Bishop

¹Hyphenated Mass Spectrometry Laboratory (HyMaS),University of Technology Sydney, ²School of Life Sciences,University of Technology Sydney

Capitoli

00:05Introduction
00:44Raw Sample Preparation
01:56Cell Lysing and Fractionation of Free Non-Protein Amino Acids (NPAAs)
03:36Fractionation of the Protein-Bound Non-Protein Amino Acids (NPAAs)
06:11Sample Filtering
06:35Results: Extraction of NPAAs from Biological Matrices via TCA Protein Precipitation and Acid Hydrolysis
07:15Conclusion

Summary

Traduzione automatica

本协议描述了在使用液相色谱-串联质谱分析之前通过三氯乙酸（TCA）蛋白质沉淀和酸水解从生物基质中提取非蛋白质氨基酸。

Tags

Keywords: Non-protein Amino Acids Cyanobacteria Liquid Chromatography-tandem Mass Spectrometry BMA AEG 2 4-DAB Extraction Freeze Drying Sonication Centrifugation Trichloroacetic Acid

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

规模以上非靶向代谢组学剖析血清超高效液相色谱 - 质谱（UPLC-MS）

不相关的代谢组学，生物源使用超高效液相色谱 - 高分辨质谱（UPLC-HRMS）

利用毛细管电泳从植物组织量化有机酸：一个测试用例检查<em>小粒种咖啡</em>种子

洞察氨基酸和多肽与无机材料的使用相互作用的单分子力谱

液相色谱离子迁移 QTOF 质谱测定蛋白质生物中宿主细胞蛋白的 HS-MRM 法

简捷方法对硫甙的提取与分析用高压液相色谱法（HPLC）

挥发性有机化合物与大气压化学电离四极杆质谱联用

利用二次 Nanoelectrospray 电离耦合高分辨质谱的实时呼吸分析

寡 peptoids 的合成及质谱分析

基于毛细管电泳的氢/氘交换，用于自上而下的质谱法对蛋白质进行构象表征

Read Article