Rivista
/
Bioingegneria
/
Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models
JoVE Journal
Bioingegneria
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Bioingegneria
Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models

Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models

DOI:
10.3791/63791-v

•

10:49 min

•

June 16, 2022

•

, , , , , , , , , , , , , , , ,
1University of California Los Angeles

Capitoli

  • 00:05Introduction
  • 00:50Preparation of Hydrogel Solution
  • 02:32Illumination and Photocrosslinking of Hydrogels via an LED Array
  • 04:20Setting Up and Drug Treatment of 3D, Matrix-Embedded Cultures
  • 08:22Results: Effect of HA Based 3D Miniaturized Cultures on Matrix Stiffness and Integrin-Binding Peptides
  • 10:24Conclusion

Summary

Traduzione automatica

Traduzione automatica

The present protocol describes an experimental platform to assess the effects of mechanical and biochemical cues on chemotherapeutic responses of patient-derived glioblastoma cells in 3D matrix-mimetic cultures using a custom-made UV illumination device facilitating high-throughput photocrosslinking of hydrogels with tunable mechanical features.

Tags

Keywords: 3D Tumor ModelsHydrogel ArraysHigh-throughput ScreeningMatrix ComponentsTherapeuticsGBM CellsDrug ResistanceHEPES-buffered SolutionThiolated Hyaluronic AcidPEG NorbornenePEG ThiolCysteine-thiol-containing PeptidesUV LED Illumination

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/63791?article_type=v

Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology
check_url/it/63791?article_type=v

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
check_url/it/63791?article_type=v

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
check_url/it/63791?article_type=v

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation
check_url/it/63791?article_type=v

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel
check_url/it/63791?article_type=v

Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs
check_url/it/63791?article_type=v

A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk
check_url/it/63791?article_type=v

Fabrication of Ti3C2 MXene Microelectrode Arrays for In Vivo Neural Recording
check_url/it/63791?article_type=v

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion
check_url/it/63791?article_type=v

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

Read Article