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Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures
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Capitoli
- 00:04Introduction
- 01:06Embryo Incubation and Preparation of Culture Dishes
- 01:49Isolation of Chick Embryos
- 03:01Dissecting Neural Folds
- 04:22Plating Neural Folds
- 05:20Fixing and Staining of Cultured Migratory Neural Crest Cells for Morphological Analysis
- 07:08Results: Assessment of Cultured Migratory Neural Crest Cells
- 08:39Conclusion
This versatile protocol describes the isolation of premigratory neural crest cells (NCCs) through the excision of cranial neural folds from chick embryos. Upon plating and incubation, migratory NCCs emerge from neural fold explants, allowing for assessment of cell morphology and migration in a simplified 2D environment.
