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Developmental Biology

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JoVE Journal Developmental Biology
Forberedelse og morfologisk analyse af chick kraniale neurale crestcellekulturer
 
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Forberedelse og morfologisk analyse af chick kraniale neurale crestcellekulturer

Article DOI: 10.3791/63799-v • 09:26 min • June 27th, 2022
June 27th, 2022 •
1, 2,3, 2,3, 2,3
1Department of Biology and Neuroscience Program, Hamline University, 2Department of Genetics, Cell Biology and Development, University of Minnesota, 3Developmental Biology Center, University of Minnesota

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Denne alsidige protokol beskriver isoleringen af præmigratoriske neurale kamceller (NCC'er) gennem udskæring af kraniale neurale folder fra kyllingembryoner. Ved plettering og inkubation kommer migrerende NCC'er ud af neurale foldeksplanter, hvilket giver mulighed for vurdering af cellemorfologi og migration i et forenklet 2D-miljø.

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Udviklingsbiologi udgave 184
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