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Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches

Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches

DOI:
10.3791/63807-v

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05:23 min

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May 20, 2022

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, , , , , , , , , ,
1Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences

Capitoli

  • 00:05Introduction
  • 00:43Perfusion, Triple Fluorescent Staining, and Tissue Clearing Treatment
  • 03:10Results: Enhanced Understanding of the Skin Innervation via Immunofluorescence and a 3D View of the Cutaneous Nerve Fibers in the Hairy and Glabrous Skin
  • 04:31Conclusion

Summary

Traduzione automatica

Traduzione automatica

The thickness of tissue sections limited the morphological study of the skin innervation. The present protocol describes a unique tissue clearing technique to visualize cutaneous nerve fibers in thick 300 µm tissue sections under confocal microscopy.

Tags

Fluorescent StainingSkin InnervationCGRPLYVE-13D ImagingConfocal Microscopy

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