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1Department of Development and Regeneration, Woman and Child Unit, CF research lab,KU Leuven, 2Department of Pediatrics, Pediatric Pulmonology,University Hospitals Leuven, 3VIB Bio Imaging Core,VIB-KU Leuven Center for Brain & Disease Research, 4Department for Neuroscience,KU Leuven, 5Interuniversity Center for Biostatistics and Statistical Bioinformatics,University of Leuven and University of Hasselt, 6Department of Pediatrics, 2nd Faculty of Medicine,Charles University and Motol University Hospital, 7Department of Chronic Diseases and Metabolism (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID),KU Leuven, 8Department of Development and Regeneration, Stem Cell Institute Leuven (SCIL),KU Leuven, 9Department of Gastroenterology and Hepatology,University Hospitals Leuven, KU Leuven, 10Department of Chronic Diseases, Metabolism and Ageing; Pneumology,KU Leuven, 11Department of Respiratory Diseases,University Hospitals Leuven
Capitoli
- 00:04Introduction
- 00:59Organoid Imaging Using Confocal Microscopy
- 02:49Image Analysis and Measuring the Indices in the Imaging Software
- 05:55Results: Morphological Characteristics Determination Using Rectal Organoid Morphology Analysis
- 07:13Conclusion
Этот протокол описывает анализ морфологии ректальных органоидов (ROMA), новый диагностический анализ муковисцидоза (МВ). Морфологические характеристики, а именно округлость (индекс циркулярности, CI) и наличие светового потока (коэффициент интенсивности, IR), являются мерой функции CFTR. Анализ 189 испытуемых показал идеальную дискриминацию между муковисцидозом и без муковисцидоза.
