È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:04Introduction
- 00:59Assembly of the Microscopy Chamber
- 02:33Imaging Microtubule Bundles with 3‐Color TIRF
- 03:41Performing Optical Trap Experiments on Microtubule Bundles
- 06:02Results: Measuring Force Production by Kinesin‐5 and Frictional Force by PRC1
- 07:06Conclusion
Tags
Microtubule BundlesCytoskeletal NetworksForce MeasurementOptical TrapTIRF MicroscopySingle-molecule ExperimentsProtein ConcentrationProtein DensityMitosisNeural DevelopmentMuscle ContractionCell MigrationStreptavidinNeutrAvidinCaseinBiotinylated MicrotubulesRhodamine MicrotubulesRigor Kinesin-coated BeadsReaction Buffer
