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由人诱导多能干细胞改造的同基因肾小球芯片
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells
DOI:

10:23 min

November 04, 2022

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Capitoli

  • 00:04Introduction
  • 00:59Preparation of Microfluidic Organ Chip Devices
  • 01:31Seeding of viECs and Intermediate Mesoderm Cells into the Microfluidic Device
  • 07:15Podocyte Induction and Chip Maintenance
  • 08:03Results: Differentiation of viECs and Podocytes on Organ-On-A-Chip Device
  • 09:35Conclusion

Summary

Traduzione automatica

这里介绍的是一种设计个性化器官芯片系统的协议,该系统通过整合与人类诱导的多能干细胞分化的遗传匹配的上皮和血管内皮细胞来概括肾小球过滤屏障的结构和功能。这种生物工程系统可以推进肾脏精准医疗和相关应用。

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