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Isolering af helcelleproteinlysater fra musens ansigtsprocesser og dyrkede palatale mesenchymceller til fosfoproteinanalyse
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
DOI:

07:26 min

April 01, 2022

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Capitoli

  • 00:04Introduction
  • 00:48Harvesting E11.5 Mouse Embryos
  • 02:31Dissecting Maxillary Processes from E11.5 Mouse Embryos
  • 04:12Isolating Whole Cell Protein Lysates from Mouse Maxillary Processes
  • 05:06Isolating Whole Cell Protein Lysates from Primary and/or Immortalized Mouse Embryonic Palatal Mesenchyme Cells
  • 06:22Results: Assessing Phosphorylated Protein Levels in Cell Lysates from Cultured Mouse Embryonic Palatal Mesenchyme Cells
  • 06:59Conclusion

Summary

Traduzione automatica

Protokollen præsenterer en metode til isolering af helcelleproteinlysater fra dissekerede museembryo-ansigtsprocesser eller dyrkede museembryonale palatale mesenchymceller og udfører efterfølgende western blotting for at vurdere fosforylerede proteinniveauer.

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