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Faire progresser l’imagerie haute résolution des assemblages de virus dans le liquide et la glace
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1Department of Biomedical Engineering,Pennsylvania State University, 2Department of Materials Science and Engineering,McMaster University, 3Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, 4Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, 5Materials Research Institute,Pennsylvania State University, 6Huck Institutes of the Life Sciences,Pennsylvania State University, 7Applications team,Direct Electron, 8Application Scientist,Protochips, Inc., 9Department of Biology,Wake Forest University
Capitoli
- 00:05Introduction
- 01:43Loading the Specimen Holder for Liquid-EM
- 02:47Production of Microchip Sandwich Assemblies
- 03:51Imaging Specimens Using a Transmission Electron Microscope
- 05:15Analysis of SARS-CoV-2 Sub-Viral Assemblies in Liquid
- 06:09Results: Representative structures of AAV, SARS-CoV-2 Sub-Viral Assemblies and Rotavirus DLPs Prepared in Liquid and Vitreous Ice Using the Microchip Sandwich Technique
- 07:24Conclusion
