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High-Throughput Screening of Microbial Isolates with Impact on Caenorhabditis elegans Health
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Biologia
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JoVE Journal Biologia
High-Throughput Screening of Microbial Isolates with Impact on Caenorhabditis elegans Health

High-Throughput Screening of Microbial Isolates with Impact on Caenorhabditis elegans Health

DOI:
10.3791/63860-v

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11:40 min

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April 28, 2022

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, , ,
1Division of Biomedical and Life Sciences,Lancaster University

Capitoli

  • 00:05Introduction
  • 01:03C. elegans Culturing on OP50
  • 04:05Preparing Gut Microbiota Isolate Collections for Re-Feeding Worms
  • 05:14LFASS Heat Shock and Oxidative Assay Setup
  • 07:49Data Inspection
  • 08:26LFASS Data Processing
  • 10:02Results: High-Throughput Screening of Bacterial Isolates for Impact on Nematode Stress Resistance
  • 10:47Conclusion

Summary

Traduzione automatica

Traduzione automatica

Gut microbes may positively or negatively impact the health of their host via specific or conserved mechanisms. Caenorhabditis elegans is a convenient platform to screen for such microbes. The present protocol describes high-throughput screening of 48 bacterial isolates for impact on nematode stress resistance, used as a proxy for worm health.

Tags

Keywords: High-throughput ScreeningCaenorhabditis ElegansOxidative Thermal Stress ResistanceBacterial IsolatesNematode ModelsDrug ScreeningProbioticsHost-microbe InteractionsMetabolic DisordersStress HandlingAging

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