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Optisk fangst af plasmoniske nanopartikler til in situ overfladeforstærkede Raman-spektroskopikarakteriseringer
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

Optisk fangst af plasmoniske nanopartikler til in situ overfladeforstærkede Raman-spektroskopikarakteriseringer

DOI:
10.3791/63862-v

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06:19 min

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June 23, 2022

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, ,
1HKUST-Shenzhen Research Institute, 2Department of Chemistry,The Hong Kong University of Science and Technology

Capitoli

  • 00:04Introduction
  • 01:00Optical Setup
  • 01:47Preparation of Sample Chamber and Generation of AgNp Assembly for SERS Measurement
  • 04:13Results: SERS Spectra of the Analyte Molecules Enhanced by the Plasmonic AgNp Assembly
  • 05:32Conclusion

Summary

Traduzione automatica

Traduzione automatica

Den nuværende protokol beskriver en bekvem tilgang til integration af optisk fangst og overfladeforbedret Raman-spektroskopi (SERS) til at manipulere plasmoniske nanopartikler til følsom molekylær detektion. Uden aggregeringsmidler samler fangstlaseren plasmoniske nanopartikler for at forbedre SERS-signalerne fra målanalytter til di situ-spektroskopiske målinger.

Tags

Keywords: Optical TrappingPlasmonic NanoparticlesSurface-enhanced Raman SpectroscopySERS-active Nanoparticle AssemblyAnalyte DetectionBiomarker DetectionMicrofluidic System532-nanometer LaserLiquid-nitrogen-cooled Charge-coupled Device CameraSilver NanoparticlesDSNB SolutionRaman Excitation Source Laser

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