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Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography
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JoVE Journal Immunologia e infezioni
Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography

Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography

DOI:
10.3791/63895-v

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00:00 min

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May 19, 2022

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, ,
1Mycobacteria Research Laboratories, Department of Microbiology Immunology & Pathology,Colorado State University, 2BioMARC, Infectious Disease Research Center,Colorado State University

Capitoli

  • 00:04Introduction
  • 00:50Preparation of Crude Mtb EV Concentrate
  • 02:36Size Exclusion Chromatography for the Enrichment of Mtb EVs from CFP
  • 05:52Quantification and Qualification of the Mtb EVs
  • 07:25Results: Silver Stain and Western Blots by Fraction, TEM Images, and NTA Distribution
  • 08:42Conclusion

Summary

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This protocol describes size exclusion chromatography, a facile and reproducible technique for enriching Mycobacterium tuberculosis extracellular vesicles from culture supernatants.

Tags

Keywords: Mycobacterium TuberculosisExtracellular VesiclesSize Exclusion ChromatographyEnrichmentUltracentrifugationCentrifugal FilterPBSBCA AssaySize Exclusion Chromatography ColumnAFC Tower

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