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Karakterisering af mekaniske egenskaber ved primær cellevæg i levende planteorganer ved hjælp af atomkraftmikroskopi
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Biologia
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JoVE Journal Biologia
Characterizing Mechanical Properties of Primary Cell Wall in Living Plant Organs Using Atomic Force Microscopy

Karakterisering af mekaniske egenskaber ved primær cellevæg i levende planteorganer ved hjælp af atomkraftmikroskopi

DOI:
10.3791/63904-v

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09:52 min

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May 18, 2022

•

,
1Laboratory of Plant Cell Growth Mechanisms,Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS

Capitoli

  • 00:04Introduction
  • 00:35Sample Preparation for Atomic Force Microscopy Measurements
  • 03:06Data Acquisition
  • 06:40Data Evaluation and Post-Processing
  • 07:26Results: Investigation of Primary Cell Walls of Plant Organs Using Atomic Force Microscopy
  • 09:04Conclusion

Summary

Traduzione automatica

Traduzione automatica

Undersøgelser af cellevægsbiomekanik er afgørende for at forstå plantevækst og morfogenese. Følgende protokol foreslås at undersøge tynde primære cellevægge i det indre væv i unge planteorganer ved hjælp af atomkraftmikroskopi.

Tags

Plant Cell WallCell GrowthBiomechanical CharacterizationAtomic Force MicroscopyVibratome SectioningAgarosePlant OrganNon-fixedNon-dehydratedCell Wall Properties

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