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Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart
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JoVE Journal Bioingegneria
Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart

Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart

DOI:
10.3791/63926-v

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08:20 min

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May 19, 2022

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, , ,
1Department of Physiology and Biophysics,Case Western Reserve University, 2University of California Los Angeles (UCLA) Cardiac Arrhythmia Center,David Geffen School of Medicine, 3UCLA Neurocardiology Research Program of Excellence,UCLA

Capitoli

  • 00:04Introduction
  • 00:27Capacitive Immunoprobe Fabrication and Functionalization
  • 03:24Experimental Setup for In Vitro Detection and Measurement of Peptide
  • 04:41Adaptation of the Capacitive Immunoprobe for In Vivo Use
  • 05:56Results: Assessment of Capacitive Immunoprobe

Summary

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Traduzione automatica

Established immunochemical methods to measure peptide transmitters in vivo rely on microdialysis or bulk fluid draw to obtain the sample for offline analysis. However, these suffer from spatiotemporal limitations. The present protocol describes the fabrication and application of a capacitive immunoprobe biosensor that overcomes the limitations of the existing techniques.

Tags

Time-resolved MeasurementNeuropeptide DynamicsCapacitive ImmunoprobePorcine HeartIn VivoPlatinum WireDopaminePoly-dopamine DepositionAntibody SolutionFlow Chamber

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