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通过电容式免疫探 针在猪 心中对神经肽动力学进行时间分辨体内测量

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Bioingegneria

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JoVE Journal Bioingegneria

Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart

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通过电容式免疫探针在猪心中对神经肽动力学进行时间分辨体内测量

DOI:

10.3791/63926-v

•

08:20 min

•

May 19, 2022

•

Nicholas Kluge, Shyue-An Chan, Jeffrey L. Ardell³, Corey Smith

¹Department of Physiology and Biophysics,Case Western Reserve University, ²University of California Los Angeles (UCLA) Cardiac Arrhythmia Center,David Geffen School of Medicine, ³UCLA Neurocardiology Research Program of Excellence,UCLA

Capitoli

00:04Introduction
00:27Capacitive Immunoprobe Fabrication and Functionalization
03:24Experimental Setup for In Vitro Detection and Measurement of Peptide
04:41Adaptation of the Capacitive Immunoprobe for In Vivo Use
05:56Results: Assessment of Capacitive Immunoprobe

Summary

Traduzione automatica

已建立的免疫化学方法 在体内 测量肽递质，依靠微透析或体液抽取来获得样品进行离线分析。但是，这些都受到时空限制。本方案描述了克服现有技术局限性的电容式免疫探针生物传感器的制造和应用。

Tags

Keywords: Time-resolved Measurement Neuropeptide Dynamics Capacitive Immunoprobe Porcine Heart In Vivo Platinum Wire Dopamine Poly-dopamine Deposition Antibody Solution Flow Chamber

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