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1Walter-Brendel-Centre of Experimental Medicine,University Hospital, LMU Munich, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 3Division of Experimental Cardiology, Department of Cardiology, Erasmus MC,University Medical Center Rotterdam, 4Institute of Cellular and Molecular Physiology,Friedrich-Alexander-Universität Erlangen-Nürnberg, 5Muscle Research Center Erlangen (MURCE),Friedrich-Alexander-Universität Erlangen-Nürnberg, 6Department of Cardiac Surgery,University Hospital, LMU Munich
Capitoli
- 00:04Introduction
- 01:01Preparing Agarose and Vibratome
- 02:01Trimming and Embedding the Samples
- 03:39Placing the Samples on Cutting Tray and Setting Vibratome
- 04:17Medium and Incubator Preparation During Slicing Procedure
- 05:07Preparing and Mounting Slices
- 07:06Changing Medium
- 07:51Results: Ex Vivo Cultivation of Human Left Ventricular Myocardial Tissue for Detailed Analysis of Contraction Forces and Kinetics
- 10:15Conclusion
Wir präsentieren ein Protokoll für die Ex-vivo-Kultivierung von menschlichem ventrikulärem Myokardgewebe. Es ermöglicht eine detaillierte Analyse der Kontraktionskraft und Kinetik sowie die Anwendung von Vor- und Nachlast, um die in vivo physiologische Umgebung genauer nachzuahmen.
