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从未受干扰和受伤的小鼠骨骼肌中分离和培养纤维脂肪祖细胞和肌肉干细胞的FACS-分离和培养
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle
DOI:

11:25 min

June 08, 2022

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Capitoli

  • 00:04Introduction
  • 01:04Hind Limb Muscle Harvesting
  • 03:11Mechanical and Enzymatic Muscle Digestion
  • 05:04Generation of Mononucleated Cells
  • 07:03Antibody Staining for Flow Cytometry
  • 08:49Results: FACS-Assisted Simultaneous Isolation and Culture of FAPs and MuSCs from Mouse Limb Muscles
  • 10:37Conclusion

Summary

Traduzione automatica

纤维脂肪祖细胞(FAPs)和肌肉干细胞(MuSCs)的精确鉴定对于研究它们在生理和病理条件下的生物学功能至关重要。该协议为从成年小鼠肌肉中分离,纯化和培养FAP和MuSC提供了指南。

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