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In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes
DOI:

08:20 min

August 17, 2022

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Capitoli

  • 00:04Introduction
  • 00:58Experimental Malaria Infection in Mouse
  • 01:49Obtaining Fresh Whole Mouse Splenocytes
  • 03:30Cytotoxic Effector Cell Purification: CD8a+ T Cell Negative Selection
  • 04:22P. yoelii Infected RBC Isolation
  • 05:21CFSE Labeling of RBCs, Preparation for Flow Cytometry and Cytotoxic Lymphocyte Coculture
  • 06:22Results: P. yoelii – iRBC Enrichment and Assessment of cell lysis Percentage of P. yoelii – iRBCs by CD8+ lymphocytes
  • 07:39Conclusion

Summary

Traduzione automatica

Here we describe a new method to help elucidate the mechanisms of cellular immunity to Plasmodium during the blood stage of infection. This is an in vitro assay that measures infected red blood cell killing by cytotoxic lymphocytes. 

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